Scientist I, Molecular Biology and Expression, Protein Sciences - ref. d70353623

Scientist I Molecular Biology and Expression, Protein Sciences

April 2024

Work Location: Corporate Headquarters (South San Francisco)

Position Summary

The successful candidate will have extensive skills in molecular biology and experience in expression and purification of recombinant proteins using both transient and stable systems. As part of an interdisciplinary research team, the selected individual will be a key contributor to the progression of an exciting and diverse therapeutic pipeline.

• Perform molecular biology techniques including high throughput PCR and molecular cloning, and from small to large scale plasmid DNA purification.
• Design and generate recombinant antigen protein expression plasmid for bacterial and mammalian expression systems.
• HTP transient expression of antibodies in mammalian cell lines on automated platform.
• Develop and generate antibody stable expression in CHO and 293.
• Accurately and consistently record experimental methods, materials, and results.
• Provide regular progress updates and present data at internal team meetings and departmental meetings.

• M.S. in Molecular Biology, Chemical Biology, Biochemistry or other relevant discipline with 5+ years of relevant experience, or B.S. with equivalent experience.
• Self-motivated and passionate about automation and new technology.
• Extensive hands-on experience with prime design and PCR.
• Experienced with various molecular cloning methods such as seamless and GoldenGate cloning is required.
• Extensive hands-on experience with transient mammalian expression (HEK293 and CHO) is required.
• Hands-on experience with automation using liquid handler is highly desired.
• Experience with recombinant protein expression in bacterial and/or other expression system is highly desired, especially good understanding on solubility enhancing techniques.
• Hands-on experience with stable mammalian expression (HEK293 and CHO) is desired.
• Hands-on experience of mono- and bi-specific antibodies, Fab fragments, scFv, VHH, and various antibody-fusion formats is desired.
• Strong organizational skills and ability to handle multiple projects simultaneously.
• Comfortable to work in a fast-paced work environment.

Company Overview

This model supports scientific entrepreneurs from the initial idea to clinical proof of concept (PoC) by combining a dedicated fund (TCG Labs) with our independent, evergreen R&D center led by a top-tier scientific team (Soleil). Together, we create an integrated ecosystem that fosters the development of single-asset portfolio companies, strategically poised for transacting after clinical PoC.

For more details, visit TCGLSoleil.com and follow us on LinkedIn

EEO Statement

Any request for accommodation can be directed to contact@tcglsoleil.com.

Benefits and Compensation

TCG Labs-Soleil provides a competitive benefits package that includes access to Medical, Dental, and Vision Insurance, Life Insurance, Short and Long Term Disability, Health Savings Account (HSA), Flexible Spending Accounts (FSA), 401k plan and PTO.

The anticipated salary range for fully qualified candidates applying for this role will be $98,000 - $171,875 annually (not including discretionary bonus, equity, and benefits). The final salary offered will be dependent on several factors that include but are not limited to relevant previous experience including duration and type of experience within the industry, education, and internal equity.

Staffing Agencies

Inquiries must be sent to contact@tcglsoleil.com with the Subject Heading: Staffing Agency Request.